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Question
The complete digestion of a plasmid with EcoRI and HindIII would give rise to which of the following fragments?
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Solution
The correct option is B 200 kb, 300 kb, and 475 kb
The given plasmid has two EcoRI sites and one Hind III sites. Digestion of this plasmid with these two enzymes will produce total three fragments as EcoRI will make two cuts and will make two fragments of 200 kb and 775 kb. HindIII will make the single cut at the location of its restriction site in 775 kb fragment and will produce two fragments of 300 kb and 475 kb. In total, three fragments of 200 kb, 475 kb and 300 kb. Therefore, the correct answer is option B.
The given plasmid has two EcoRI sites and one Hind III sites. Digestion of this plasmid with these two enzymes will produce total three fragments as EcoRI will make two cuts and will make two fragments of 200 kb and 775 kb. HindIII will make the single cut at the location of its restriction site in 775 kb fragment and will produce two fragments of 300 kb and 475 kb. In total, three fragments of 200 kb, 475 kb and 300 kb.
Therefore, the correct answer is option B.
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