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Gram Staining

The component...

Question

The component of bacteria that retains the crystal violet stain during Gram-staining is

A

O-antigen

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B

Lipopolysaccharide

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C

Peptidoglycan

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D

Cytoplasmic membrane

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Solution

The correct option is C Peptidoglycan
Gram staining includes staining of both gram positive and gram negative bacterial cell purple with crystal violet followed by stabilization of dye in a peptidoglyacan layer with the mordant. The presence of thick peptidoglycan layer in gram positive cells makes them retain the dye after alcohol treatment while gram negative bacteria become colorless. The correct answer is option C.

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