Question

The correct labelling for the given image is

• A. 1- HRP, 2- conjugated secondary antibody, 3- primary antibody
• B. 1- HRP, 2- Primary antibody, 3- Conjugated secondary antibody
• C. 1- Primary antibody, 2- HRP, 3- Conjugated secondary antibody
• D. None of the above

Answer 1

The correct option is A 1- HRP, 2- conjugated secondary antibody, 3- primary antibody

The primary antibody (3) binds to the antigen which is to be detected in the ELISA method. The conjugated secondary antibody (2) is attached to the HRP (1) which is an enzyme. The secondary antibody binds to the primary antibody. When this reaction occurs and the substrate is added, HRP acts on it to produce the product which results in change in the colour.

Thus, the correct answer is option A.