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Question
The correct sequence in the tissues culture technique is
The correct sequence in the tissues culture technique is
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Solution
The correct option is B Preparation of medium - inoculation - explant incubation - callus formation- organogenesis -transferred to field
- The tissue culture technique involves many steps. The first step of tissue culture is the preparation of medium.
- For this, growth medium in the form of broth or agar is used containing appropriate nutrients. The mother plant is selected and is inoculated in the medium.
- After the inoculation, the explant is incubated under proper condition of temperature under sterilized environment.
- The unorganized tissue mass grows on the medium which is called as callus. Organogenesis involves two distinct phases dedifferentiation and redifferentiation.
- The process of dedifferentiation starts after the isolation of the explant. Redifferentiation starts after the first callus cell forms followed by organogenesis. The plant is then transferred to the field.
Thus, the correct answer is option B.
- The tissue culture technique involves many steps. The first step of tissue culture is the preparation of medium.
- For this, growth medium in the form of broth or agar is used containing appropriate nutrients. The mother plant is selected and is inoculated in the medium.
- After the inoculation, the explant is incubated under proper condition of temperature under sterilized environment.
- The unorganized tissue mass grows on the medium which is called as callus. Organogenesis involves two distinct phases dedifferentiation and redifferentiation.
- The process of dedifferentiation starts after the isolation of the explant. Redifferentiation starts after the first callus cell forms followed by organogenesis. The plant is then transferred to the field.
Thus, the correct answer is option B.
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