The correct sequence of different steps of polymerase chain reaction is

Annealing denaturation extension

No worries! We‘ve got your back. Try BYJU‘S free classes today!

Denaturation extension annealing

No worries! We‘ve got your back. Try BYJU‘S free classes today!

Denaturation annealing extension

Right on! Give the BNAT exam to get a 100% scholarship for BYJUS courses

Extension denaturation annealing

No worries! We‘ve got your back. Try BYJU‘S free classes today!

Open in App

Solution

The correct option is B Denaturation annealing extension
Polymerase chain reaction is a technique used to replicate a fragment of DNA so as to produce many copies of a particular DNA sequence. A single PCR amplification cycle involves three basic steps: denaturation, annealing and extension (polymerisation).
So, the correct answer is 'Denaturation annealing extension'.

Suggest Corrections

Similar questions

	A	B	C	D
a	Denaturation at 94-96 degree C	Extension through Taq polymerase at 72 degree C	Repetition of denaturation and annealing	Annealing at 72 degree C
b	Denaturation at 94-96 degree C	Annealing at 40-60 degree C	Extension through Taq polymerase at 72 degree C	Repetition of denaturation and annealing
c	Denaturation at 40-60 degree C	Annealing at 72 degree C	Extension through Taq polymerase at 94-96 degree C	Repetition of denaturation and annealing
d	Extension through Taq polymerase at 72 degree C	Denaturation at 40 degree C	Annealing at 72 degree C	Repetition of denaturation and annealing

\begin{matrix} Column I & Column II (a) & Thermus aquaticus & (i) & Denaturation, annealing, extension (b) & Polymerase chain reaction & (ii) & Thermostable DNA polymerase (c) & Plasmid & (iii) & Post-transcriptional silencing (d) & Meloidogyne incognita & (iv) & Cloning vector \end{matrix}

\begin{matrix} PCR steps & Temperature P. Denaturation & i. 72^{\circ} C Q. Annealing & ii. 92 - 96^{\circ} C R. Extension & iii. 55 - 65^{\circ} C \end{matrix}

Join BYJU'S Learning Program