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Question

The dyes that are used in Gram's staining are

A
Crystal violet and methylene blue
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B
Crystal violet and safranin
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C
Crystal violet and carbol fuchsin
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D
Safranin and methylene blue
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Solution

The correct option is B Crystal violet and safranin
The Gram's staining involves three processes: staining with a water-soluble dye called crystal violet, decolorization, and counterstaining, usually with safranin. Due to differences in the thickness of a peptidoglycan layer in the cell membrane between Gram positive and Gram negative bacteria, Gram positive bacteria (with a thicker peptidoglycan layer) retain crystal violet stain during the decolorization process, while Gram-negative bacteria lose the crystal violet stain and are instead stained by the safranin in the final staining process.

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