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Question
The factor that can reduce the enzyme activity is
I. Increasing pH level
II. Decreasing temperature
III. Placing enzyme in a nonpolar liquid
The factor that can reduce the enzyme activity is
I. Increasing pH level
II. Decreasing temperature
III. Placing enzyme in a nonpolar liquid
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Solution
The correct option is E I, II, and III
Enzymes enhance the rate of reaction by lowering down the activation energy of transition state but themselves remain unchanged. Increasing temperature will change the active site of an enzyme by breaking the weak bonds like hydrogen bond and hinders the activity. Any change in pH above or below the optimum pH affects the rate of reaction by changing the shape of the active site of an enzyme. An enzyme is a protein which functions in aqueous solution i.e., water (polar). Placing enzyme in the nonpolar liquid will alter its tertiary structure as the hydrophobic amino acid will move to the surface instead and reduce the enzymatic activity.Thus, the correct answer is E.
Enzymes enhance the rate of reaction by lowering down the activation energy of transition state but themselves remain unchanged. Increasing temperature will change the active site of an enzyme by breaking the weak bonds like hydrogen bond and hinders the activity. Any change in pH above or below the optimum pH affects the rate of reaction by changing the shape of the active site of an enzyme. An enzyme is a protein which functions in aqueous solution i.e., water (polar). Placing enzyme in the nonpolar liquid will alter its tertiary structure as the hydrophobic amino acid will move to the surface instead and reduce the enzymatic activity.Thus, the correct answer is E.
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