Question

The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving
correct identification together with what it represents ?

• A. A - Denaturation at a temperature of about ${50}^{0}C$
• B. C - Extension in the presence of heat stable DNA polymerase
• C. A - Annealing with two sets of primers
• D. B - Denaturation at a temperature of about ${98}^{0}C$ separating the two DNA strands

Answer 1

The correct option is B C - Extension in the presence of heat stable DNA polymerase

$\text{Heat stable DNA polymerase. Heat stability is very important because}$$\text{PCR reaction is performed at various temperatures.}$$\text{This heat-stable DNA polymerase is obtained from thermophilic bacteria Thermus aquaticus,}$$\text{the inhabitant of hot springs}$