The first report of successful production of haploid plants from 'anther culture' in Datura innoxia, came from the work of
A
Steward and White
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B
Nitzcli and White
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C
Skoog and Murashige
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D
Guha and Maheshwari
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Solution
The correct option is C Guha and Maheshwari
Guha and Maheshwari developed an 'anther culture' technique for the production of haploids in the laboratory. The technique was discovered in 1964 by Guha and Maheshwari. This technique can be used in over 200 species, including tomato, rice, tobacco, barley, and geranium. In experiments using Datura innoxia, induction frequencies of almost 100% and a yield of more than one thousand plantlets or calluses have occurred under optimal conditions from one anther. Success can be determined within 24 hours as cells begin to divide. A doubled haploid (DH) is a genotype formed when haploid cells undergo chromosome doubling. Artificial production of doubled haploids is important in plant breeding.