The correct option is B Polio virus
The virus lacks metabolism of its own and due to this reason, they cannot multiply without infecting a living cell. Due to this reason, viruses could not be cultured in a laboratory. In 1941 John Enders, Frederick Robbins and Thomas Weller succeeded in culturing the virus that causes polio. They cultured the poliovirus in human muscle and tissue in a laboratory setting. This was the first virus cultured in human cells. So, the correct answer is 'Poliovirus'.