Question

The given flow chart depicts the steps to transfer a desirable gene of interest into a plant.Isolation of desirable gene using restriction endonucleases and gel electrophoresis.
Regeneration of plants from the transformed cells to get transgenic plants.
Identify the missing steps (A, B and C) with regard to following statements and select the correct option.(i) Joining of desirable gene to a suitable cloning vector using ligases to create a recombinant DNA molecule(ii) Selection of transformed cells(iii) Transferring the recombinant DNA molecules to the target cells

• A. A - i
  B - ii
  C - iii
• B. A - i
  B - iii
  C - ii
• C. A - ii
  B - iii
  C - i
• D. A - iii
  B - i
  C - ii

Answer 1

Answer: (B)

A - i

B - iii
C - ii

Steps to transfer a desirable gene of interest into a plant:

• Isolation of desirable gene using restriction endonucleases and gel electrophoresis.
  
• Joining of desirable gene to a suitable cloning vector using ligases to create a recombinant DNA molecule.
• Transferring the recombinant DNA molecules to the target cells.
  
• Screening of cells.
• Transformation.
• Selection of transformed cells.
  
• Regeneration of plants from the transformed cells to get transgenic plants.
  

So, the correct option is 'Option B' .