Question

The introduction of t-DNA into plants involves

• A. Allowing the plant roots to stand in water
• B. Infection of the plant by Agrobacterium tumefaciens
• C. Altering the pH of the soil, then heat shocking the plants
• D. Exposing the plants to cold for a brief period

Answer 1

The correct option is B Infection of the plant by Agrobacterium tumefaciens

t-DNA or transfer DNA is found inside the Ti plasmid of Agrrobacterium tumefaciens. Agrobacterium is a prokaryote which infects the dicot plants. They are called natural genetic engineers.
These bacteria can insert its Ti plasmid or Tumour-inducing plasmid into the host plant and results in tumour-like growth called gall in the plants.