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Question

The linking of antibiotic resistance gene with plasmid vector became possible with:

A
DNA polymerase
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B
Exonuclease
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C
DNA ligase
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D
Endonuclease
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Solution

The correct option is C DNA ligase
The linking of antibiotic resistance gene with the plasmid vector became possible with the enzyme DNA ligase, which acts on cut DNA molecules and joins their ends. This makes a new combination of circular autonomously replicating DNA created in vitro and is known as recombinant DNA.

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