The linking of antibiotic resistance gene with the plasmid vector became possible with:
A
DNA polymerase
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B
Exonucleases
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C
DNA ligase
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D
Endonucleases
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Solution
The correct option is C DNA ligase DNA ligase is an enzyme that is able to join together two portions of DNA and therefore plays an important role in DNA repair. DNA ligase is also used in recombinant DNA technology. DNA polymerase is an enzyme that play a role in the synthesis of DNA strands on a suitable template. Exonucleases are nucleases that cut off nucleotides from the 5’ or 3’ terminal ends of DNA. Endonucleases are nuclease that cleave DNA duplexes at any point except the terminal ends.