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Question
The microbial density in a liquid broth can be estimated by which of the following technique?
The microbial density in a liquid broth can be estimated by which of the following technique?
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Solution
The correct option is B Spectrophotometer
The increase in the cell size and cell mass during the development of an organism is termed as growth. This increase in the cell mass of the organism is measured by using the spectrophotometer. The spectrophotometer measures the turbidity or optical density. Turbidity is the measure of the amount of light absorbed by a bacterial suspension. The degree of turbidity in the broth culture is directly proportional to the number of microorganism present i.e. microbial density. Increasing turbidity of the broth medium indicates an increase in the microbial cell mass. The amount of transmitted light through turbid broth decreases with subsequent increase in the absorbance value. Thus the correct answer is option A.
The increase in the cell size and cell mass during the development of an organism is termed as growth. This increase in the cell mass of the organism is measured by using the spectrophotometer. The spectrophotometer measures the turbidity or optical density. Turbidity is the measure of the amount of light absorbed by a bacterial suspension. The degree of turbidity in the broth culture is directly proportional to the number of microorganism present i.e. microbial density. Increasing turbidity of the broth medium indicates an increase in the microbial cell mass. The amount of transmitted light through turbid broth decreases with subsequent increase in the absorbance value. Thus the correct answer is option A.
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