The correct option is A To measure the exact effect of that factor on photosynthesis
To determine the effect of any factor like CO2, sunlight, chlorophyll, on photosynthesis of the plant, the plant will be kept in a dark room or cupboard for 48 hours to eliminate the starch from the leaves of the plant, which was previously formed through photosynthesis. This process is known as destarching. After destarching, the effect of that factor on the formation of starch can be easily studied.