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Question

The problem of necrosis and gradual senescence while performing tissue culture can overcome by

A
Suspension culture
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B
Subculture
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C
Spraying auxins
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D
Spraying cytokinins
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Solution

The correct option is B Subculture
Necrosis and gradual senescence are caused due to faster depletion of food and ethylene production in culture. As the biomass grow their is depletion of food, and increase in the growth regulators e.g ethylene. Some plants are sensitive to ethylene, so it is suggested to subculture before necrosis occurs. Thus the correct answer is option B.

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