The correct option is B Spraying cytokinins
Cytokinin is a plant growth regulator. It is a plant hormone. The first natural cytokinin was isolated from unripe maize grains or kernels known as zeatin. Cytokinins occur in regions of rapid cell division such as root apices, developing shoot buds, young fruits, etc. Cytokinins delay the senescence (ageing) of leaves and other organs by controlling protein synthesis and mobilization of nutrients as resources. The problem of nectosis and gradual senescence, while performing tissue culture can be overcome by spraying cytokinins.