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The process of separation and purification of expressed proteins from the cells grown on a large-scale, before marketing is called
The process of separation and purification of expressed proteins from the cells grown on a large-scale, before marketing is called
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Solution
The correct option is B downstream processing
Recombinant DNA technology is the usage of technology to alter the genome of a living cell for obtaining the desired product.
The host cell carrying the recombinant DNA undergoes transcription and translation to produce the desired recombinant protein.
For commercial purposes, host cells carrying the recombinant DNA are grown at a large scale in large vessels known as bioreactors, under regulated conditions. This is known as upstream processing or biosynthetic stage. As the number of cells in the culture media increases exponentially, the amount of desired protein expressed by them also increases. Once the concentration or density of the product expressed by the host cells in the culture reaches the optimal quantity, it needs to be extracted out for use.
The process involving separation of the expressed gene product from the culture media and its purification is known as downstream processing. It involves techniques such as filtration, centrifugation, sedimentation, chromatographic techniques, etc.
The sum total of upstream and downstream processing is known as bioprocessing.
Biotechnology is the technology of using living organisms or their parts for obtaining desired products, which can be employed for commercial purposes. Bioprocessing, including both upstream and downstream processing are components of biotechnology.
Recombinant DNA technology is the usage of technology to alter the genome of a living cell for obtaining the desired product.
The host cell carrying the recombinant DNA undergoes transcription and translation to produce the desired recombinant protein.
For commercial purposes, host cells carrying the recombinant DNA are grown at a large scale in large vessels known as bioreactors, under regulated conditions. This is known as upstream processing or biosynthetic stage. As the number of cells in the culture media increases exponentially, the amount of desired protein expressed by them also increases. Once the concentration or density of the product expressed by the host cells in the culture reaches the optimal quantity, it needs to be extracted out for use.
The process involving separation of the expressed gene product from the culture media and its purification is known as downstream processing. It involves techniques such as filtration, centrifugation, sedimentation, chromatographic techniques, etc.
The sum total of upstream and downstream processing is known as bioprocessing.
Biotechnology is the technology of using living organisms or their parts for obtaining desired products, which can be employed for commercial purposes. Bioprocessing, including both upstream and downstream processing are components of biotechnology.
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