The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is called
The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is called
The correct option is A Elution
Agarose gel electrophoresis
Agarose gel electrophoresis is the technique involving the separation of molecules based on their size and charge.
Process of Agarose gel electrophoresis
Process of Agarose gel electrophoresis is as follows
- The DNA fragments and size markers are loaded onto the gel well and current is passed through the gel.
- The DNA being negatively charged, moves towards the positive electrode during gel electrophoresis.
- The smaller the size of the molecule, the faster and farther it travels and the fragments are separated.
Resolution
Degree of separation of the different lengths of DNA fragments by the sieving effect of the agarose gel
Elution
Elution is the method by which the DNA bands, after electrophoresis, are cut along with the gel and then extracted from the gel pieces.
Spooling
DNA spooling is the removal of the precipitated DNA strand using the glass rod or spatula from the chilled ethanol after separating it from the cell
Final answer
(c) Elution
Agarose gel electrophoresis
Agarose gel electrophoresis is the technique involving the separation of molecules based on their size and charge.
Process of Agarose gel electrophoresis
Process of Agarose gel electrophoresis is as follows
- The DNA fragments and size markers are loaded onto the gel well and current is passed through the gel.
- The DNA being negatively charged, moves towards the positive electrode during gel electrophoresis.
- The smaller the size of the molecule, the faster and farther it travels and the fragments are separated.
Resolution
Degree of separation of the different lengths of DNA fragments by the sieving effect of the agarose gel
Elution
Elution is the method by which the DNA bands, after electrophoresis, are cut along with the gel and then extracted from the gel pieces.
Spooling
DNA spooling is the removal of the precipitated DNA strand using the glass rod or spatula from the chilled ethanol after separating it from the cell
Final answer
(c) Elution
