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What is PCR

The specific ...

Question

The specific palindromic sequence which is recognized by EcoRI is

A

5’- CTTAAG -3’, 3’GAATTC - 5’

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B

5’- GGATCC - 3’, 3’- CCTAGG - 5’

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C

5’- GAATTC - 3’, 3’ -CTTAAG - 5’

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D

5’ - GGAACC - 3’, 3’ - CCTTGG - 5’

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Solution

The correct option is C 5’- GAATTC - 3’, 3’ -CTTAAG - 5’
EcoRI is the restriction enzyme which recognises 6 basepair palindromic sequence and cuts both the strands of DNA at

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Q. Ratio between triple and double H-bond nucleotide base pairs in a palindrome recognized by EcoRI.

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Q. Assertion: Restriction enzymes recognise specific palindromic sequences.
Reason: Palindromic sequences are DNA sequences that are read the same way when the orientation is kept the same in both the strands.

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