Question

The steps of biopsy are listed below:
(a) Extraction of suspected tissue
(b) Cutting the tissue into thin slices
(c) Viewing it under the microscope

Why is it necessary to slice the extracted tissue so thin?

• A. The sample extracted is short in supply
• B. It fits into the slide better
• C. Many slide samples can be obtained
• D. It allows the electrons to pass right through the sample.

Answer 1

The correct option is D It allows the electrons to pass right through the sample.

When working with an electron microscope, great care needs to be taken to slice the tissue samples very thin. This allows the electrons to pass through the sample without much obstruction. With the thinness of the slicing varying from 100nm or lesser, electron microscopes have the ability to locate and detect objects that are approximately one-twentieth of a nanometer.