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Question
The sticky ends facilitates the action of the enzyme __________.
The sticky ends facilitates the action of the enzyme __________.
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Solution
The correct option is B DNA Ligase
Sticky ends are produced due to activity of restriction endonuclease. Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome sites, but between the same two bases on the opposite strands. This leaves single stranded portions at the ends. There are overhanging stretches called sticky ends on each strand. These are named so because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase during ligation with desired DNA.
Sticky ends are produced due to activity of restriction endonuclease. Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome sites, but between the same two bases on the opposite strands. This leaves single stranded portions at the ends. There are overhanging stretches called sticky ends on each strand. These are named so because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase during ligation with desired DNA.
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