Question

The tumor inducing (Ti) plasmid has now been modified into a cloning vector which is no more pathogenic to the plants but is still able to use the mechanisms to deliver genes of our interest into a variety of plants because Ti plasmid has been modified by :

• A. Deleting tumor forming genes
• B. Adding tumor forming genes
• C. Deleting endonuclease
• D. Adding genes resistant to endonucleases

Answer 1

The correct option is A Deleting tumor forming genes

The tumor inducing (Ti) plasmid of Agrobacterium tumefaciens has now been modified into a cloning vector which is no more pathogenic to the plants but is still able to use the mechanisms to deliver genes of our interest into a variety of plants because Ti plasmid has been modified by deleting tumor forming genes.

So, once a gene or a DNA fragment has been ligated into a suitable vector it is transferred into a bacterial, plant or animal host (where it multiplies).

So, the correct answer is "option D"