The UV spectroscopy is dependent on which of the following law?
A
Beer- Lambert Law
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B
Hardy-Weinberg law
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C
Wein displacement law
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D
None of the above
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Solution
The correct option is A Beer- Lambert Law
The UV spectroscopy is dependent on Beer Lambert law.
The law states that the absorbance of a solution is directly proportional to the concentration of the absorbing species in the solution and the path length. Thus, for a fixed path length, UV spectroscopy can be used to determine the concentration of a particular compound (biomolecules) that absorbs light of a specific wavelength present in a solution.