The value of numerical aperature of the objective lens of a microscope is 1.25. If light of wavelength 5000˚A is used, the minimum separation between two points, to be seen as distinct, will be :
A
0.24μm
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B
0.48μm
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C
0.12μm
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D
0.38μm
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Solution
The correct option is A0.24μm Numerical aperature of the microscope is given as NA=.0.61λd Where d= minimum sparation between two points to be seen as distinct d=0.61λNA=(0.61)×(5000x10m−10)1.25 =2.4×10−7m =0.24μm