Question

The vector plasmid and the gene of interest that have been cut with the same restriction enzyme creating sticky ends are joined by .

• A. DNA ligase
• B. DNA polymerase
• C. restriction enzyme
• D. PCR

Answer 1

The correct option is A DNA ligase

DNA ligase joins the gene of interest to the vector to form recombinant DNA. To ligate the gene of interest with the vector, sticky ends that are complimentary to the ends of the gene fragment are present in the plasmid vector. Thereafter, by the action of the enzyme DNA ligase, the ends are joined and the gene gets inserted into the vector. DNA ligase can also join blunt ends.