Question

Tissue culture medium is sterilised by

• A. Antifungal agents
• B. Keeping at -20${}^o$C
• C. Autoclaving at 120${}^o$C for 15 minutes
• D. Filtering through fine sieve

Answer 1

Answer: (C)

Autoclaving at 120${}^o$C for 15 minutes

Tissue culture is the growth of cells or tissues separated from an organism. This is facilitated using a liquid, semi-solid medium which is sterilized so as to prevent the contamination by autoclaving them. It is a process whereby applying heat and pressure, microbes are killed in a container.

So, the correct answer is 'Autoclaving at 120-degree Celsius for 15 minutes'