To determine the BOD5 of a waste water sample 10, 50, 75 and 100 ml samples of the waste water were diluted to 500 ml and incubated at 20∘C in BOD bottles for 5 days. The results were as follows:
S.NoWaste water volume, mlInital DO, mg/lDo after 5 days, mg/l1109.86.92508.64.53.758.21.24.1008.00.0
Based on the above data, the average BOD5 of the waste water sample is
BOD in mg/l = [Initial Do - Final DO] × Dilution factor
For sample 1, [BOD5]1=(9.8−6.9)×50010=145mg/l
For sample 2, [BOD5]2=(8.6−4.5)×50050=41mg/l
For sample 3, [BOD5]3=(8.2−1.2)×50075=46.67mg/l
Sample 4 not considered as its D.Ofinal=0 and so, it is uncertain whether D.O. was used up in 5 days or 2 days or 3 days.
∴ Average BOD5=145+41+46.673=77.56mg/l