Question

Ultrastructure of cell can be best studied by

• A. Autoradiography
• B. X-ray diffraction method
• C. Phase contrast microscope
• D. None of these

Answer 1

The correct option is D None of these

• Autoradiography is the production of a 2D or 3D image on a photographic film or plate by radioactive radiation. A good example is the autoradiography of nucleic acids separated by gel electrophoresis.
• X-ray diffraction is a technique used for the study of crystal structures and atomic spacing. In the X-ray diffraction method, X-rays are diffracted by electrons. The analysis of X-ray diffraction data sets produces an electron density map of the crystal.
• Phase-contrast microscopy is used for viewing unstained cells growing in tissue culture and for testing cell and organelle preparations for lysis.
• Electron microscopy is used when the greatest resolution is required, and when the living state can be ignored. The images produced in an electron microscope reveal the ultrastructure of cells.

Thus, the correct answer is option D.