What are recognition sequences of endonucleases?

Open in App

Solution

Recognition sequences of endonucleases:
Recognition sequences are the 4 to 8 nucleotide long sequences that restriction enzymes identify and are distinguished by a specific form of internal symmetry.
The recognition sequence site is recognized by restriction endonucleases and they cleave the DNA at or near the recognition site.
You may find the recognition sequences for some of the most popular restriction endonucleases in the table below:
Enzyme
Organism
Recognition Sequence
Blunt or Sticky End
EcoRI
Escherichia Coli
GAATTC
Sticky
BamHI
Bacillus amyloliquefaciens
GFATCC
Sticky
BglII
Bacillus globigii
AGATCT
Sticky
PvuI
Proteus vulgaris
CGATCG
Sticky
PvuII
Proteus vulgaris
CAGCTG
Blunt
HindIII
Haemophilus influenzae Rd
AAGCTT
Sticky
Hinfl
Haemophilus influenzae Rf
GANTC
Sticky
Sau3A
Staphylococcus aureus
GATC
Sticky
AluI
Arthrobacter luteus
AGCT
Blunt
TaqI
Thermus aquaticus
TCGA
Sticky
HaeIII
Haemophilus aegyptius
GGCC
Blunt
NotI
Nocardia otitidis-caviarum
GCGGCCGC
Sticky

Suggest Corrections