Question

What are the three main steps involved in the construction of a transgene?

Answer 1

Transgenesis is the process of the introduction of the desired gene (Transgene) from one organism into the genome of another. There are two types of transgenic organisms:

1. Transgenic animals
2. Transgenic plants

To make a transgene three steps are involved:

• Promoters - They are located in a gene at an upstream position and helps in the initiation of transcription of any gene.
• Gene to be expressed - It is the desired gene which we want to express in the organism of our choice. It is the coding region which is to be expressed.
• Termination sequence - It is the sequence or codons which codes for stopping of transcription or translation. It helps in capping and tailing of mRNA, thus maintains mRNA stability.