Glycolysis is a set of events that converts six-carbon glucose into two three-carbon keto-acids (pyruvate).
Glycolysis liberates free energy for the synthesis of high-energy molecules like ATP and NADH.
Glycolysis is divided into two phases: the energy-investment phase and the energy-yielding phase.
The energy-yielding phase of glycolysis produces ATP and NADH in the second phase.
In the first phase, glyceraldehyde-3-phosphate dehydrogenase with NAD catalyzes the conversion of D-glyceraldehyde 3-phosphate to glyceric acid 1,3-bisphosphate, which also creates NADH.
The process that transforms glyceric acid 1,3-bisphosphate to 3-phosphoglyceric acid produces ATP.
Phosphoglycerate mutase 2 converts 3-phosphoglyceric acid to 2-phospho-D-glyceric acid, while alpha-enolase with cofactor magnesium converts 3-phosphoglyceric acid to 2-phospho-D-glyceric acid.
Phospho-D-glyceric acid is converted to phosphoenolpyruvic acid.
Eventually, plastidial pyruvate kinase 4 transforms phosphoenolpyruvic acid to pyruvate with the help of cofactors magnesium and potassium, as well as ADP.
Pyruvate will proceed via pyruvate metabolism, tyrosine metabolism, pantothenate biosynthesis, and CoA biosynthesis.