Question

What is meant by cloning vector?

Answer 1

Cloning vector:

1. A cloning vector is a DNA molecule that consists of DNA fragments incorporated into it and can replicate it inside itself to produce multiple clones of recombinant DNA.
2. Plasmids and phages are the two types of vectors utilized in cloning.

Characteristics of the plasmid vector.

1. Replication's Beginnings - ORI refers to the nucleotide sequence of a DNA molecule that serves as that of the replication process's origin.
2. When foreign DNA is integrated or connected to this sequence, it begins reproducing alongside the host cell.
3. Selectable marker gene - A selectable marker gene is required in the cloning vector because it allows for the selection of host cells that contain the recombinant DNA and the separation of those that do not.
4. Restriction sites - This would have a consensus sequence to allow restriction endonuclease to break up particular sequences.