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Question

What is 'Ori'? State it's importance during cloning of a vector. [2 Marks]

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Solution

'Ori' or the origin of replication is the sequence where the replication starts in a plasmid. [1 Mark]

During cloning of a vector, it is important because any sequence linked to 'ori' in a plamid vector will get replicated. Ori controls the copy number of the linked DNA. [1 Mark]

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