Question

What is PCR involved in?

Answer 1

PCR:

1. Polymerase chain reaction or PCR is an amplification technique.
2. It has the ability to amplify small samples of DNA at a large scale.
3. PCR is involved in many research labs, forensics, genetic testing, and also in diagnostic fields.
4. PCR is a process of 20-40 repeat thermal changes called thermal cycles.
5. Each cycle consists of three different temperatures.

Steps in PCR:

1. Denaturation: At temperature between 94°-95°C, denaturation of DNA occur.
2. Parent DNA breaks and forms two strands due to the application of high temperature.
3. Annealing: At temperatures between 50°-56° C, DNA primers bind to separated stands.
4. Extending: At a temperature of 72° C, Taq polymerase bind to primer and forms double-stranded DNA from a single strand.