Question

What is Quantitative PCR?

Answer 1

Quantitative PCR (qPCR):

1. Quantitative PCR (qPCR) is a technique for detecting, characterizing, and quantifying nucleic acids in a variety of situations.
2. RNA transcripts are often measured in RT-qPCR by first reverse transcribing them into cDNA, as explained above, and then performing qPCR.
3. DNA is amplified in three phases, as in regular PCR: denaturation, annealing, and elongation.
4. In qPCR, however, fluorescent labelling allows data to be collected while the PCR progresses.
5. Due to the variety of methodologies and chemistries available, this technology provides numerous advantages.
6. Fluorescent labelling facilitates the measurement of amplified DNA molecules using a dsDNA binding dye in dye-based qPCR (usually green).
7. Many targets can be identified simultaneously in each sample using probe-based qPCR, but this needs the optimization and creation of target-specific probe(s) in addition to primers.