Question

What is the difference between trypsin and chymotrypsin?

Answer 1

Trypsin:

1. It is a digestive enzyme released by the pancreas as trypsinogen that breaks down proteins in the small intestine.

Chymotrypsin:

1. It is a digestive enzyme that aids in the breakdown of proteins in the small intestine.
2. The pancreas secretes it, and trypsin converts it to an active form.

Difference between trypsin and chymotrypsin:

Trypsin	Chymotrypsin
1). The two kinds are $\mathrm{\alpha }$- and $\mathrm{\beta }$-trypsin.	1). The two kinds are chymotrypsin A and B.
2). Peptide bonds at the C-terminus of lysine or arginine are hydrolyzed.	2). Peptide bonds at the C-terminus of tryptophan, phenylalanine and tyrosine are hydrolyzed.
3). DFP, Ag+, aprotinin, EDTA, benzamidine, and other inhibitors are available.	4). Hydroxymethylpyrroles, courmarin derivatives, boronic acids, peptidyl aldehydes, and other inhibitors are available.
5). Tissue dissociation, mitochondrial isolation, cell harvesting,in vitro protein research, and other applications.	5). Sequence analysis, peptide mapping, peptide synthesis, peptide fingerprinting, and other applications.