Question

What is the direction of synthesis of DNA?

• A. 3' to 5'
• B. 5' to 3'
• C. Varies in eukaryotes
• D. Varies in prokaryotes

Answer 1

The correct option is B 5' to 3'

• DNA synthesis takes place by the enzyme DNA dependent DNA polymerase which catalyzes polymerization only in one direction, which is 5' to 3' direction.
• On 3' to 5' strand of DNA, DNA polymerase works in 5' to 3' direction in a discontinuous manner and creates fragment that are later joined by the DNA ligase enzyme.

So, the correct answer is option B.