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PCR in Diagnosis

What is the f...

Question

What is the function of a restriction enzyme in recombinant technology?

A

Cut DNA into many fragments

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B

Link together newly joined fragments of DNA

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C

Make millions of copies of a specific segment of DNA

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D

Separate fragments of DNA by their length and electrical charges

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Solution

The correct option is B Cut DNA into many fragments
Restriction enzymes are DNA-cutting enzymes. Each enzyme recognizes one or a few target sequences and cuts DNA at or near those sequences. They are also called molecular scissors.
So, the correct option is 'Cut DNA into many fragments'.

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Similar questions

Q. Identify the correct sequence/steps involved in recombinant DNA technology:
A) Culturing the host cells in a medium at large scale
B) Isolation of DNA
C) Isolation of a desired DNA fragment
D) Transferring the recombinant DNA into the host
E) Ligation of the DNA fragment into a vector
F) Fragmentation of DNA by restriction endonucleases
G) Extraction of the desired product

Q. A.......... is a collection of DNA fragments, produced by restriction-enzymes and incorporated into plasmids.

Q. How many of the following statements are correct?
I. If a linear DNA molecule is digested with a restriction enzyme at four recognition sites, 4 fragments would be produced.
II. If a closed circular DNA molecule is digested with a restriction enzyme at six recognition sites, 6 fragments would be produced.
III. If a linear DNA molecule is digested with a restriction enzyme at four recognition sites on the DNA, 5 fragments would be produced.
IV. If a closed circular DNA molecule is digested with a restriction enzyme at six recognition sites, 7 fragments would be produced.

Q. A molecular biologist used restriction enzyme for digesting a specific sequence of DNA, which molecular biology procedure he would be using to separate the DNA fragments?

Q. Consider the following steps during recombinant DNA technology.
a. Breaking of donor DNA using restriction enzyme
b. Culture of cloned bacteria containing the fragment of donor DNA
c. Isolation of suitable plasmid
d. Insertion of recombinant DNA through cloning
e. Joining of donor DNA with a suitable plasmid using ligase
The correct sequence of these is?

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