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Purification of Colloidal Solution

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What is the meaning of blunt/flush/sticky ends?

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Q. Assertion: Sticky ends forms hydrogen bonds with their complementary cut counterparts.
Reason: In recombinant DNA technology, sticky end producing restriction enzymes are preferred over blunt ends producing restriction enzymes.

Q. Which of the following enzyme creates a homonucleotide overhang at the

3^{'}

-OH end of the DNA? It can also make blunt ends sticky by adding poly AA and poly TT.

Q. How many of the following statements regarding restriction enzymes are correct?
A. They generally recognise palindromic nucleotide sequences.
B. They are endonucleases.
C. They are isolated from viruses.
D. A particular restriction enzyme produces the same kind of sticky or blunt ends in any DNA molecules.

Q. A recombinant DNA is formed when sticky ends of vector DNA and foreign DNA join. Explain how the sticky ends are formed and get joined.

Q. The sticky ends facilitates the action of the enzyme __________.

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