What will be the effect if pBR322, a cloning vector does not carry 'ori site?

Sticky ends will not produce

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Transformation will not take place

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The cell will transform into a tumour cell

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Replication will not take place

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Solution

The correct option is B Replication will not take place
For the multiplication of any alien piece of DNA in an organism, it needs to be a part of a chromosome which has a specific sequence known as 'origin of replication' (ori). If ori is not present in a cloning vector, replication will not be initiated.

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