Question

What would happen if the DNA(transgene) integrates after one or two cell divisions post pronuclear microinjection of gene in a mice?

• A. No transgenic mice will be produced
• B. The transgene will be present in all the cells
• C. The transgene will fail to produce the specific protein
• D. The resulting mouse will contain both transfected as well as non-transfected cells

Answer 1

The correct option is D The resulting mouse will contain both transfected as well as non-transfected cells

In pronucleus microinjection, transgene is injected into the male pronucleus. It is more common for the DNA to integrate after one or two cell divisions, in such cases, the resulting mouse is a mosaic containing both transfected and non-transfected cells. However, if those cells contribute to the germ line of the embryo, transgenic gametes will be produced and the subsequent generation of animals will be transgenic.