The correct option is A Disappear
Fresh water protozoans live in hypotonic solution. Therefore, to regulate the excess water which comes into the protoplasm through the process of endosmosis, contractile vacuoles are formed.
When these protozoans are placed in marine water i.e., hypertonic water, the cell start to lose water through the process of exosmosis. Therefore, due to this loss of water, the contractile vacuoles disappear inside the cell.