Question

When the following amino acids are separated by running them on Agarose gel at pH-7, which one of them will migrate slowest to anode end?

• A. Aspartic acid
• B. Valine
• C. Glycine
• D. Lysine

Answer 1

The correct option is D Lysine

At pH-7 amino acid bear no charge i.e. zero change called as zwitterion structure.

Aspartic acid is -ve charged

Valine is -ve charged

Glycine is -ve charged

Lysine is +ve charged

When the following amino acids are separated by running them on Agarose gel at pH-7 negatively charged amino acids move towards the anode (+ve pole) and positively charged amino acids move towards the cathode (-ve pole).

Since lysine bears +ve charge it moves very slowly towards anode end due to repulsion.

So the answer is 'Lysine'.