Question

Which of the following statements is correct in the context of observing DNA separated by agarose gel electrophoresis?

• A. DNA can be seen in visible light
• B. Ethidium bromide stained DNA can be seen under exposure to UV light
• C. Ethidium bromide stained DNA can be seen in visible light
• D. DNA can be seen without staining in visible light

Answer 1

The correct option is B Ethidium bromide stained DNA can be seen under exposure to UV light

Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) for techniques such as agarose gel electrophoresis. When the DNA stained with ethidium bromide is exposed to ultravoilet light, it will fluoresce with an orange colour.