Question

Which bonds are formed by DNA ligase?

Answer 1

Bonds formed by DNA ligase:

1. Phosphodiester bonds are formed by DNA ligase.
2. DNA ligases are enzymes that can establish a phosphodiester bond between a 3′-OH group and a 5′-monophosphate at a single-strand break in DNA.
3. By connecting breaks in the phosphodiester backbone of DNA that occur during replication and recombination, as well as as a result of DNA damage and repair, DNA ligases serve an important role in maintaining genomic integrity.
4. DNA ligase works by forming two covalent phosphodiester bonds between the 3′ hydroxyl end of one nucleotide (acceptor) and the 5′ phosphate end of another (donor).
5. Each phosphodiester bond requires the use of two ATP molecules.