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pBR322

How the genes...

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How the genes inserted to vector plasmid gets integrated to host chromosome? Does DNA ligase forms sugarphosphate bond also?

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Q. Assertion (A): DNA ligase plays an essential role in recombinant DNA technology.
Reason (R): The linking of antibiotic resistance genes with plasmid vectors became possible by enzyme DNA ligase.

Q. From the below list, which of the following is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
(i) Transformbacteria with recombinant DNA molecule.
(ii) Cut the plasmid DNA using restriction enzymes.
(iii) Extract plasmid DNA from bacterial cells.
(iv) Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
(v) Use ligase to seal plasmid DNA to nonplasmid DNA.

Q. During gene cloning, the enzyme used to join the insert DNA with the plasmid vector is

Q. Identify the correct sequence of steps in the construction of recombinant DNA.

(1)

use restriction enzymes

(2)

(3)

remove plasmid from parent bacterium

(4)

introduce plasmid into new host bacterium.

Q. Assertion: Ti plasmids are used to transfer genes to the host cells
Reason: They are natural vectors which deliver genes to the eukaryotic cells

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