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Ligase

During gene c...

Question

During gene cloning, the enzyme used to join the insert DNA with the plasmid vector is

A

DNA ligase

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B

Restriction endonuclease

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C

Alkaline phosphatase

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D

Exonuclease

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Solution

The correct option is B DNA ligase
The process of cloning starts with the cleavage of foreign DNA by a restriction endonuclease enzyme.
The cleaved DNA is ligated to the plasmid vector for its transformation into the host cell.
The DNA is ligated by DNA ligase by the formation of phosphodiester bonds.

So, the correct answer is option A.

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