Following steps are involved in cloning a eukaryotic gene in a bacterial plasmid vector.
I. Introduction of cloning vector into cells.
II. Insertion of eukaryotic DNA into the vector.
III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).
All of the following statements is true about restriction enzymes except
DNA fragments to be cloned in DNA vector can be fragmented by using restriction enzymes. Restriction enzymes are specific to bacteria which recognize 4-8 base pair specific sequences present in DNA. The site where restriction enzymes act are called as a restriction site. As these enzymes cleave DNA inside the molecule, these enzymes are also called restriction endonucleases. Thus, the correct answer is option B.